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產品詳(xiang)情
簡(jian)單介(jie)紹:
重組馬幹擾(rao)素(su)-γ蛋白與其(qi)它公司(si)提(ti)供的重(zhong)組蛋白(bai)不(bu)同,rEqIFN-γ蛋(dan)白(bai)產品為采(cai)用(yong)CFS的無(wu)細胞麥(mai)胚(pei)蛋白合(he)成系(xi)統表達(da)出(chu)來的重(zhong)組蛋白(bai),可(ke)表達(da)出(chu)對細胞有(you)毒(du)性(xing)、易被蛋白(bai)酶降(jiang)解(jie)的蛋(dan)白;並(bing)獲(huo)得(de)具有良(liang)好的可(ke)溶(rong)性,並(bing)有(you)翻(fan)譯(yi)後(hou)修(xiu)飾(shi)、從(cong)而(er)部分(fen)具有功(gong)能(neng)的蛋(dan)白(bai).同時(shi)獨有的全自(zi)動(dong)蛋(dan)白(bai)純(chun)化(hua)技術(shu)則簡(jian)便(bian)高效,將蛋白(bai)純(chun)化(hua)過程(cheng)中對蛋白(bai)的損傷(shang)降低(di)到*小程(cheng)度.重組馬幹擾(rao)素(su)-γ蛋白(全長(chang)序(xu)列(lie))產品可(ke)用(yong)於Western Blot驗(yan)證(zheng)、抗體制備(bei)、蛋(dan)白檢測(ce)、ELISA等試(shi)驗中.
詳(xiang)情介紹:
重組馬幹擾(rao)素(su)-γ蛋白
| Species | Equine |
| Accession | P42160 |
| GeneID | 100034181 |
| Source | Escherichia coli. |
| Molecular Weight | 重(zhong)組馬幹擾(rao)素(su)-γ蛋白Approximately 16.7 kDa, a single non-glycosylated polypeptide chain containing 143 amino acids. |
| Quantity | 20µg/100µg/1000µg |
| AA Sequence | QAAFFKEIEN LKEYFNASNP DVGDGGPLFL DILKNWKEDS DKKIIQSQIV SFYFKLFENL KDNQVIQKSM DTIKEDLFVK FFNSSTSKLE DFQKLIQIPV NDLKVQRKAI SELIKVMNDL SPKANLRKRK RSQNPFRGRR ALQ |
| Purity | 重組馬幹擾(rao)素(su)-γ蛋白> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis (EMC) virus is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重組馬幹擾(rao)素(su)-γ蛋白Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4, with 5 % trehalose. |
| Endotoxin | Less than 0.1 EU/µg of rEqIFN-γ as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組馬幹擾(rao)素(su)-γ蛋白 | |
| SDS-PAGE |
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