重組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)

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產品(pin)名(ming)稱：重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)
產品(pin)型(xing)號：rPoIL-1RA
產品(pin)廠(chang)商：KALANG
產品(pin)文檔：

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重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)與(yu)其(qi)它(ta)公司提供(gong)的重組蛋(dan)白(bai)不(bu)同，rPoIL-1RA蛋(dan)白(bai)產品(pin)為采用(yong)CFS的無細胞(bao)麥胚(pei)蛋(dan)白(bai)合成系(xi)統(tong)表達出(chu)來(lai)的重組蛋(dan)白(bai)，可(ke)表達(da)出(chu)對(dui)細胞(bao)有毒性(xing)、易(yi)被蛋(dan)白(bai)酶降解的蛋(dan)白(bai)；並(bing)獲得具(ju)有(you)良(liang)好的可溶性(xing)，並有翻(fan)譯(yi)後修(xiu)飾、從而部分具(ju)有(you)功能的蛋(dan)白(bai).同(tong)時(shi)獨(du)有(you)的全自動(dong)蛋(dan)白(bai)純(chun)化技術則簡便(bian)高效(xiao)，將(jiang)蛋(dan)白(bai)純(chun)化過程(cheng)中(zhong)對(dui)蛋(dan)白(bai)的損傷降低到*小(xiao)程(cheng)度.重組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)（全長(chang)序(xu)列(lie)）產品(pin)可(ke)用(yong)於(yu)Western Blot驗證、抗(kang)體(ti)制(zhi)備、蛋(dan)白(bai)檢(jian)測、ELISA等(deng)試(shi)驗中(zhong).

詳(xiang)情(qing)介紹：

重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)

Species Porcine

Accession Q29056

GeneID 397499

Source Escherichia coli.

Molecular Weight 重組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

Quantity 5µg/20µg/1000µg

AA Sequence HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ

Purity 重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 75 pg/ml rPoIL-1α.

Physical Appearance 重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT.

Endotoxin Less than 0.1 EU/µg of rPoIL-1RA as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)

SDS-PAGE

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Species	Porcine
Accession	Q29056
GeneID	397499
Source	Escherichia coli.
Molecular Weight	重組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Quantity	5µg/20µg/1000µg
AA Sequence	HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ
Purity	重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 75 pg/ml rPoIL-1α.
Physical Appearance	重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT.
Endotoxin	Less than 0.1 EU/µg of rPoIL-1RA as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)
SDS-PAGE

產品(pin)名(ming)稱：重(zhong)組豬白(bai)細(xi)胞(bao)介素-1受(shou)體(ti)拮(jie)抗(kang)劑蛋(dan)白(bai)蛋(dan)白(bai)