重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白 - 上海(hai)康朗(lang)生物(wu)科(ke)技有(you)限公(gong)司(si)

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產品名(ming)稱(cheng)：重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白
產品型(xing)號：rRtSDF-1β/CXCL12β
產品廠(chang)商：KALANG
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重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白與其(qi)它(ta)公(gong)司(si)提(ti)供的重(zhong)組(zu)蛋白不同，rRtSDF-1β/CXCL12β蛋白產品為(wei)采用(yong)CFS的無(wu)細(xi)胞麥(mai)胚(pei)蛋白合(he)成系統表達(da)出(chu)來的重(zhong)組(zu)蛋白，可表達(da)出(chu)對細(xi)胞有(you)毒性(xing)、易(yi)被蛋白酶降解(jie)的蛋白；並獲(huo)得(de)具有(you)良(liang)好的可溶性(xing)，並(bing)有(you)翻譯(yi)後(hou)修飾、從而(er)部分(fen)具有(you)功能(neng)的蛋白.同時獨有(you)的全自(zi)動蛋白純化(hua)技術則簡便高效(xiao)，將蛋白純化(hua)過(guo)程中(zhong)對蛋白的損傷降低(di)到*小程度(du).重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白（全長(chang)序列(lie)）產品可用於(yu)Western Blot驗(yan)證、抗體(ti)制備、蛋白檢測(ce)、ELISA等試(shi)驗中.

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重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白

Species Rat

Accession Q9QZD1

GeneID 24772

Source Escherichia coli.

Molecular Weight 重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.

Quantity 2µg/10µg/1000µg

AA Sequence KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KM

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 100-200 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin 重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白Less than 1 EU/μg of rRtSDF-1β/CXCL12β as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白

Reference 1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.
2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.
3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.
4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.
5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.
6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.
7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.

Background CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. In all SDF-1 isoforms, SDF-1β is the canonical sequence. It has the complete amino acids in the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, murine CXCL12β shares approximately 96 % amino acid sequence identity with human CXCL12β.

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Species	Rat
Accession	Q9QZD1
GeneID	24772
Source	Escherichia coli.
Molecular Weight	重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.
Quantity	2µg/10µg/1000µg
AA Sequence	KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KM
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 100-200 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白Less than 1 EU/μg of rRtSDF-1β/CXCL12β as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白
Reference	1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500. 2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9. 3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51. 4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9. 5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23. 6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703. 7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.
Background	CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. In all SDF-1 isoforms, SDF-1β is the canonical sequence. It has the complete amino acids in the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, murine CXCL12β shares approximately 96 % amino acid sequence identity with human CXCL12β.

產品名(ming)稱(cheng)：重(zhong)組(zu)大鼠(shu)基質(zhi)細(xi)胞衍生因子(zi)-1 beta / CXCL12 beta蛋白