重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白 - 上(shang)海(hai)康(kang)朗生物(wu)科技(ji)有限(xian)公(gong)司

產(chan)品(pin)展示

產品(pin)詳(xiang)情(qing)

產(chan)品(pin)名稱：重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白
產品(pin)型號：rRtSDF-1α/CXCL12α
產(chan)品(pin)廠商：KALANG
產品(pin)文(wen)檔：

妳(ni)添加了1件商品(pin) 查(zha)看(kan)購物(wu)車

簡(jian)單(dan)介紹(shao)：

重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白與其它公司(si)提(ti)供(gong)的重(zhong)組(zu)蛋(dan)白不同(tong)，rRtSDF-1α/CXCL12α蛋(dan)白產品(pin)為采(cai)用CFS的無(wu)細(xi)胞麥胚蛋(dan)白合成(cheng)系統(tong)表達出來的重(zhong)組(zu)蛋(dan)白，可(ke)表達出對細(xi)胞有毒性、易(yi)被(bei)蛋(dan)白酶(mei)降解的(de)蛋(dan)白；並獲(huo)得具有良(liang)好(hao)的可(ke)溶(rong)性，並有翻(fan)譯(yi)後(hou)修(xiu)飾、從而(er)部分(fen)具有功能(neng)的蛋(dan)白.同時(shi)獨有的全(quan)自動(dong)蛋(dan)白純化(hua)技(ji)術則簡便高(gao)效(xiao)，將(jiang)蛋(dan)白純化(hua)過(guo)程(cheng)中(zhong)對(dui)蛋(dan)白的損(sun)傷(shang)降低到*小(xiao)程(cheng)度(du).重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白（全長序(xu)列）產品(pin)可(ke)用於Western Blot驗(yan)證、抗(kang)體(ti)制(zhi)備(bei)、蛋(dan)白檢測、ELISA等試(shi)驗(yan)中.

詳(xiang)情(qing)介(jie)紹(shao)：

重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白

Species Rat

Accession Q9QZD1

GeneID 24772

Source Escherichia coli.

Molecular Weight 重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Quantity 2µg/10µg/1000µg

AA Sequence KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rRtSDF-1α/CXCL12α as determined by LAL method.

Reconstitution 重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白

Reference 1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500.
2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9.
3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51.
4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9.
5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23.
6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703.
7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.

Background CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α.

標題(ti)：

內容(rong)：

聯(lian)系(xi)人(ren)：

聯系(xi)電(dian)話(hua)：

Email：

公(gong)司(si)名(ming)稱：

聯系(xi)地址(zhi)：

註(zhu)：1.可(ke)以使(shi)用快捷鍵(jian)Alt+S或(huo)Ctrl+Enter發送(song)信息(xi)!

2.如(ru)有必要(yao),請(qing)您留下您的詳(xiang)細(xi)聯系方式(shi)!

滬公(gong)網安(an)備(bei) 31011702004399號

Species	Rat
Accession	Q9QZD1
GeneID	24772
Source	Escherichia coli.
Molecular Weight	重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
Quantity	2µg/10µg/1000µg
AA Sequence	KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	Less than 1 EU/μg of rRtSDF-1α/CXCL12α as determined by LAL method.
Reconstitution	重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白
Reference	1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500. 2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9. 3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51. 4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9. 5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23. 6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703. 7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.
Background	CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α.

產(chan)品(pin)名稱：重(zhong)組(zu)大鼠基(ji)質細(xi)胞衍生(sheng)因(yin)子(zi)-1 alpha / CXCL12 alpha蛋(dan)白