重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai) - 上海康朗(lang)生(sheng)物(wu)科技有(you)限(xian)公(gong)司

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產品名(ming)稱(cheng)：重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)
產品型(xing)號(hao)：rRtPDGF-AA
產(chan)品廠商：KALANG
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重(zhong)組大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)與其它(ta)公(gong)司提供(gong)的(de)重組蛋白(bai)不同(tong)，rRtPDGF-AA蛋白(bai)產品為(wei)采用CFS的無細(xi)胞(bao)麥(mai)胚蛋(dan)白(bai)合成系統(tong)表(biao)達出(chu)來的(de)重組(zu)蛋(dan)白(bai)，可表(biao)達出(chu)對細(xi)胞(bao)有毒(du)性(xing)、易(yi)被(bei)蛋(dan)白(bai)酶(mei)降(jiang)解的(de)蛋白(bai)；並(bing)獲(huo)得(de)具(ju)有(you)良好的可溶(rong)性(xing)，並(bing)有(you)翻(fan)譯後修(xiu)飾(shi)、從(cong)而(er)部分(fen)具(ju)有(you)功(gong)能的蛋(dan)白(bai).同時(shi)獨(du)有的(de)全(quan)自動(dong)蛋(dan)白(bai)純(chun)化(hua)技術(shu)則(ze)簡(jian)便(bian)高(gao)效(xiao)，將(jiang)蛋(dan)白(bai)純(chun)化(hua)過程中(zhong)對蛋(dan)白(bai)的損(sun)傷降(jiang)低(di)到(dao)*小(xiao)程度(du).重(zhong)組大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)（全(quan)長序列(lie)）產品可(ke)用於Western Blot驗證、抗體(ti)制備、蛋(dan)白(bai)檢(jian)測(ce)、ELISA等試驗中(zhong).

詳(xiang)情(qing)介紹：

重(zhong)組大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)

Species Rat

Accession P28576

GeneID 25266

Source Escherichia coli.

Molecular Weight Approximately 25.3 kDa, a disulfide-linked homodimeric protein containing two 111 amino acid residues polypeptide (A chain).

Quantity 2µg/10µg/1000µg

AA Sequence 重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.

Physical Appearance 重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Endotoxin Less than 0.1 EU/μg of rRtPDGF-AA as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)

SDS-PAGE

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Species	Rat
Accession	P28576
GeneID	25266
Source	Escherichia coli.
Molecular Weight	Approximately 25.3 kDa, a disulfide-linked homodimeric protein containing two 111 amino acid residues polypeptide (A chain).
Quantity	2µg/10µg/1000µg
AA Sequence	重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.
Physical Appearance	重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Endotoxin	Less than 0.1 EU/μg of rRtPDGF-AA as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)
SDS-PAGE

產品名(ming)稱(cheng)：重組(zu)大鼠(shu)血小(xiao)板衍(yan)生(sheng)生(sheng)長因子(zi)-AA蛋(dan)白(bai)