重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai) - 上(shang)海康朗生(sheng)物(wu)科技(ji)有(you)限公(gong)司(si)

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產品(pin)名稱：重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)
產(chan)品(pin)型(xing)號(hao)：rRtPDGF-BB
產品(pin)廠(chang)商：KALANG
產(chan)品(pin)文(wen)檔：

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重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)與(yu)其它公(gong)司(si)提供的重(zhong)組蛋白(bai)不(bu)同(tong)，rRtPDGF-BB蛋白(bai)產(chan)品(pin)為(wei)采(cai)用(yong)CFS的無(wu)細(xi)胞麥(mai)胚(pei)蛋白(bai)合(he)成系(xi)統表(biao)達(da)出(chu)來的重(zhong)組蛋白(bai)，可(ke)表(biao)達(da)出(chu)對細(xi)胞有(you)毒性(xing)、易被蛋白(bai)酶(mei)降(jiang)解的蛋白(bai)；並獲(huo)得(de)具有(you)良好的可溶性(xing)，並有(you)翻(fan)譯(yi)後(hou)修(xiu)飾(shi)、從(cong)而部(bu)分具(ju)有(you)功能(neng)的蛋白(bai).同(tong)時獨有(you)的全(quan)自(zi)動(dong)蛋白(bai)純(chun)化技(ji)術(shu)則(ze)簡便高(gao)效(xiao)，將(jiang)蛋白(bai)純(chun)化過程(cheng)中對蛋白(bai)的損傷降(jiang)低到*小(xiao)程(cheng)度.重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)（全(quan)長(chang)序(xu)列）產品(pin)可(ke)用於(yu)Western Blot驗(yan)證、抗體(ti)制(zhi)備、蛋白(bai)檢(jian)測、ELISA等(deng)試驗(yan)中.

詳(xiang)情介(jie)紹：

重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)

Species Rat

Accession Q05028

GeneID 24628

Source Escherichia coli.

Molecular Weight 重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)Approximately 24.4 kDa, a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide (B chain).

Quantity 2µg/10µg/1000µg

AA Sequence SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT

Purity 重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 6.9.

Endotoxin Less than 0.1 EU/μg of rRtPDGF-BB as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)

SDS-PAGE

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Species	Rat
Accession	Q05028
GeneID	24628
Source	Escherichia coli.
Molecular Weight	重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)Approximately 24.4 kDa, a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide (B chain).
Quantity	2µg/10µg/1000µg
AA Sequence	SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT
Purity	重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 6.9.
Endotoxin	Less than 0.1 EU/μg of rRtPDGF-BB as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)
SDS-PAGE

產品(pin)名稱：重(zhong)組大(da)鼠血(xue)小(xiao)板(ban)衍生(sheng)生(sheng)長(chang)因(yin)子(zi)-BB蛋白(bai)