重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai) - 上(shang)海(hai)康朗生物(wu)科(ke)技有限(xian)公(gong)司(si)

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產品(pin)名(ming)稱：重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)
產品(pin)型(xing)號：rRtHB-EGF
產品(pin)廠商(shang)：KALANG
產品(pin)文(wen)檔：

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重組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)與(yu)其(qi)它公(gong)司(si)提(ti)供(gong)的重(zhong)組(zu)蛋白(bai)不(bu)同(tong)，rRtHB-EGF蛋白(bai)產品(pin)為(wei)采用CFS的無(wu)細(xi)胞(bao)麥胚(pei)蛋白(bai)合成(cheng)系(xi)統表達(da)出(chu)來的(de)重組(zu)蛋白(bai)，可表達(da)出(chu)對(dui)細胞(bao)有毒(du)性、易被蛋(dan)白酶降(jiang)解(jie)的(de)蛋白(bai)；並(bing)獲得(de)具(ju)有(you)良好(hao)的(de)可溶(rong)性，並(bing)有翻(fan)譯後修飾(shi)、從(cong)而(er)部(bu)分(fen)具(ju)有(you)功能(neng)的(de)蛋(dan)白.同時獨有的全自(zi)動蛋(dan)白(bai)純化(hua)技術(shu)則簡便高(gao)效(xiao)，將(jiang)蛋白(bai)純化(hua)過程中對(dui)蛋白(bai)的損(sun)傷降(jiang)低(di)到(dao)*小程度(du).重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)（全(quan)長(chang)序列）產品(pin)可(ke)用於(yu)Western Blot驗證、抗(kang)體制備(bei)、蛋白(bai)檢(jian)測(ce)、ELISA等試驗(yan)中.

詳情介(jie)紹：

重組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)

Synonyms HBEGF, DT-R

Species Rat

Accession Q06175

GeneID 25433

Source Escherichia coli.

Molecular Weight 重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

Quantity 10µg/50µg/1000µg

AA Sequence DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose.

Endotoxin 重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)Less than 1 EU/μg of rRtHB-EGF as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)

Reference 1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73.
2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.

Background Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Rat HB-EGF shares about 76 % a.a. sequence identity with human HB-EGF.

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Synonyms	HBEGF, DT-R
Species	Rat
Accession	Q06175
GeneID	25433
Source	Escherichia coli.
Molecular Weight	重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
Quantity	10µg/50µg/1000µg
AA Sequence	DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose.
Endotoxin	重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)Less than 1 EU/μg of rRtHB-EGF as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)
Reference	1. Jin K, Mao XO, Sun Y, et al. 2002. J Neurosci. 22:5365-73. 2. Higashiyama S, Abraham JA, Miller J, et al. 1991. Science. 251:936-9.
Background	Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Rat HB-EGF shares about 76 % a.a. sequence identity with human HB-EGF.

產品(pin)名(ming)稱：重(zhong)組(zu)大(da)鼠肝素(su)結(jie)合性EGF樣(yang)生長(chang)因(yin)子(zi)蛋(dan)白(bai)