重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai) - 上(shang)海(hai)康(kang)朗(lang)生物科(ke)技(ji)有(you)限公司(si)

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產(chan)品名(ming)稱：重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)
產(chan)品型號(hao)：rRtVEGF164
產(chan)品廠商：KALANG
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重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)與其(qi)它(ta)公司(si)提(ti)供的重(zhong)組(zu)蛋(dan)白(bai)不同(tong)，rRtVEGF164蛋(dan)白(bai)產(chan)品為采(cai)用(yong)CFS的無(wu)細(xi)胞麥胚蛋(dan)白(bai)合(he)成(cheng)系(xi)統表(biao)達出來(lai)的重(zhong)組(zu)蛋(dan)白(bai)，可(ke)表達出對(dui)細(xi)胞有(you)毒(du)性(xing)、易被蛋(dan)白(bai)酶(mei)降(jiang)解的蛋(dan)白(bai)；並(bing)獲得(de)具有(you)良(liang)好(hao)的可(ke)溶(rong)性(xing)，並(bing)有(you)翻譯(yi)後修飾、從而(er)部分具有(you)功能的蛋(dan)白(bai).同(tong)時(shi)獨有(you)的全自動蛋(dan)白(bai)純(chun)化技(ji)術則(ze)簡便(bian)高(gao)效，將(jiang)蛋(dan)白(bai)純(chun)化過程(cheng)中(zhong)對蛋(dan)白(bai)的損傷(shang)降(jiang)低(di)到*小程(cheng)度(du).重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)（全長(chang)序(xu)列(lie)）產(chan)品可用於(yu)Western Blot驗證、抗(kang)體制(zhi)備、蛋(dan)白(bai)檢(jian)測、ELISA等試(shi)驗中(zhong).

詳情介紹：

重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)

Synonyms VPF

Species Rat

Accession P16612

GeneID 83785

Source 重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)Escherichia coli.

Molecular Weight Approximately 38.7 kDa, a disulfide-linked homodimeric protein, consisting of two 165 amino acid polypeptide chains with Met at N-terminus.

Quantity 2µg/10µg/1000µg

AA Sequence MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Purity 重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.8.

Endotoxin 重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)Less than 1 EU/μg of rRtVEGF164 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)

Reference 1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9.
2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94.
3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.

Background Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 164, and 188 amino acids (a.a.) in length. Recombinant Rat VEGF165 contains 165 amino acids residues (with an met at N- terminal) and it is a disulfide-linked homodimer. In addition, it shares 97 % a.a. sequence identity with corresponding regions of mouse, 88 % with human and bovine, 89 % with porcine and canine, and 90 % with feline and equine VEGF, respectively.

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Synonyms	VPF
Species	Rat
Accession	P16612
GeneID	83785
Source	重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)Escherichia coli.
Molecular Weight	Approximately 38.7 kDa, a disulfide-linked homodimeric protein, consisting of two 165 amino acid polypeptide chains with Met at N-terminus.
Quantity	2µg/10µg/1000µg
AA Sequence	MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Purity	重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.8.
Endotoxin	重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)Less than 1 EU/μg of rRtVEGF164 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)
Reference	1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9. 2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94. 3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.
Background	Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 164, and 188 amino acids (a.a.) in length. Recombinant Rat VEGF165 contains 165 amino acids residues (with an met at N- terminal) and it is a disulfide-linked homodimer. In addition, it shares 97 % a.a. sequence identity with corresponding regions of mouse, 88 % with human and bovine, 89 % with porcine and canine, and 90 % with feline and equine VEGF, respectively.

產(chan)品名(ming)稱：重(zhong)組(zu)大(da)鼠(shu)血(xue)管內(nei)皮(pi)生長(chang)因(yin)子(zi)164蛋(dan)白(bai)