重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白 - 上(shang)海(hai)康(kang)朗(lang)生(sheng)物科技(ji)有限公(gong)司

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產(chan)品名稱：重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白
產(chan)品型(xing)號：rRtEGF
產(chan)品廠(chang)商：KALANG
產(chan)品文(wen)檔：

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重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白與其它公司(si)提供(gong)的重組(zu)蛋白不(bu)同(tong)，rRtEGF蛋白產(chan)品為(wei)采(cai)用(yong)CFS的無(wu)細胞(bao)麥(mai)胚(pei)蛋白合(he)成(cheng)系統表達出(chu)來(lai)的重組(zu)蛋白，可表達出(chu)對(dui)細胞(bao)有毒(du)性(xing)、易被蛋白酶降解(jie)的蛋白；並獲(huo)得具(ju)有良(liang)好(hao)的可溶(rong)性(xing)，並有(you)翻(fan)譯後(hou)修(xiu)飾、從(cong)而(er)部分(fen)具(ju)有功能(neng)的蛋白.同(tong)時(shi)獨(du)有的全(quan)自動蛋白純(chun)化技術則簡便(bian)高效，將(jiang)蛋白純(chun)化過程中對(dui)蛋白的損傷降低(di)到*小程度(du).重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白（全(quan)長(chang)序列）產(chan)品可用(yong)於Western Blot驗證、抗體制(zhi)備、蛋白檢(jian)測(ce)、ELISA等試驗中.

詳(xiang)情介紹(shao)：

重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白

Synonyms Urogastrone, URG

Species Rat

Accession P07522

GeneID 25313

Source Escherichia coli.

Molecular Weight 重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白Approximately 6.1 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids, including 3 intramolecular disulfide-bonds.

Quantity 20µg/100µg/1000µg

AA Sequence NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rRtEGF as determined by LAL method.

Reconstitution 重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白

SDS-PAGE

Reference 1. Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6.
2. Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-62.
3. Yang H, Sun X, Wang Z, et al. 2003. J Membr Biol, 194: 47-58.
4. Cohen S. 2008. J Biol Chem, 283: 33793-7.

Background Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. It is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature rat EGF shares 70 % a.a. sequence identity with mature human EGF.

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Synonyms	Urogastrone, URG
Species	Rat
Accession	P07522
GeneID	25313
Source	Escherichia coli.
Molecular Weight	重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白Approximately 6.1 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids, including 3 intramolecular disulfide-bonds.
Quantity	20µg/100µg/1000µg
AA Sequence	NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rRtEGF as determined by LAL method.
Reconstitution	重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白
SDS-PAGE
Reference	1. Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6. 2. Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-62. 3. Yang H, Sun X, Wang Z, et al. 2003. J Membr Biol, 194: 47-58. 4. Cohen S. 2008. J Biol Chem, 283: 33793-7.
Background	Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. It is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature rat EGF shares 70 % a.a. sequence identity with mature human EGF.

產(chan)品名稱：重組(zu)大(da)鼠表皮(pi)生長(chang)因子(zi)蛋白