重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白 - 上海康(kang)朗(lang)生(sheng)物科(ke)技(ji)有(you)限(xian)公司(si)

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產品名(ming)稱：重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白
產品型(xing)號(hao)：rRtFGF-21
產品廠商：KALANG
產品文(wen)檔：

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重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白與(yu)其它(ta)公司(si)提供(gong)的(de)重(zhong)組蛋(dan)白不(bu)同，rRtFGF-21蛋白產品為采用(yong)CFS的無(wu)細(xi)胞麥(mai)胚蛋白合(he)成(cheng)系(xi)統(tong)表達出來的(de)重(zhong)組蛋(dan)白，可(ke)表達出對細(xi)胞有(you)毒(du)性(xing)、易(yi)被(bei)蛋白酶(mei)降(jiang)解的(de)蛋(dan)白；並(bing)獲得具(ju)有(you)良好的可溶(rong)性(xing)，並(bing)有(you)翻譯後修(xiu)飾(shi)、從(cong)而部(bu)分(fen)具有(you)功能(neng)的蛋白.同(tong)時獨有的全自動(dong)蛋白純(chun)化技(ji)術則簡(jian)便(bian)高(gao)效，將(jiang)蛋(dan)白純(chun)化過(guo)程中(zhong)對蛋(dan)白的(de)損傷降(jiang)低(di)到(dao)*小(xiao)程度.重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白（全(quan)長(chang)序列(lie)）產品可(ke)用(yong)於(yu)Western Blot驗證、抗(kang)體(ti)制備(bei)、蛋白檢(jian)測、ELISA等(deng)試驗中(zhong).

詳(xiang)情介(jie)紹：

重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白

Species Rat

Accession Q8VI80

GeneID 170580

Source Escherichia coli.

Molecular Weight 重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

Quantity 5µg/25µg/1000µg

AA Sequence AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10³ IU/mg in the presence of 5 µg/ml of rMuKlotho-β and 10 μg/ml of heparin.

Physical Appearance White lyophilized powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.

Endotoxin 重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白Less than 1 EU/µg of rRtFGF-21 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白

SDS-PAGE

Reference 1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6.
2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.
3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95

Background Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and murine FGF-21, and it show activity on murine and human cells.

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Species	Rat
Accession	Q8VI80
GeneID	170580
Source	Escherichia coli.
Molecular Weight	重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
Quantity	5µg/25µg/1000µg
AA Sequence	AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10³ IU/mg in the presence of 5 µg/ml of rMuKlotho-β and 10 μg/ml of heparin.
Physical Appearance	White lyophilized powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.
Endotoxin	重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白Less than 1 EU/µg of rRtFGF-21 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白
SDS-PAGE
Reference	1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6. 2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35. 3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95
Background	Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and murine FGF-21, and it show activity on murine and human cells.

產品名(ming)稱：重(zhong)組大(da)鼠(shu)成(cheng)纖維細胞生(sheng)長(chang)因子(zi)21蛋(dan)白