重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai) - 上海(hai)康(kang)朗生(sheng)物科技有限公(gong)司(si)

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產(chan)品名(ming)稱：重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)
產(chan)品型(xing)號：rRtFGF-18
產(chan)品廠(chang)商：KALANG
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重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)與(yu)其(qi)它公(gong)司提(ti)供(gong)的重(zhong)組(zu)蛋白(bai)不同(tong)，rRtFGF-18蛋白(bai)產(chan)品為(wei)采(cai)用CFS的無細胞(bao)麥(mai)胚(pei)蛋(dan)白合(he)成系統(tong)表(biao)達(da)出來(lai)的重(zhong)組(zu)蛋白(bai)，可(ke)表(biao)達(da)出對(dui)細(xi)胞(bao)有(you)毒性、易(yi)被(bei)蛋白(bai)酶降(jiang)解(jie)的蛋白；並獲(huo)得(de)具(ju)有(you)良好的可(ke)溶性，並(bing)有(you)翻譯後修(xiu)飾、從而部分(fen)具(ju)有功(gong)能(neng)的蛋白.同(tong)時(shi)獨(du)有的全自(zi)動(dong)蛋白(bai)純化技術(shu)則(ze)簡便高效(xiao)，將蛋白純化過(guo)程中對(dui)蛋(dan)白的損傷降(jiang)低到(dao)*小程度(du).重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)（全長序列）產品可(ke)用於Western Blot驗證(zheng)、抗體制備(bei)、蛋(dan)白(bai)檢(jian)測(ce)、ELISA等(deng)試(shi)驗中.

詳(xiang)情(qing)介紹(shao)：

重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)

Species Rat

Accession O88182

GeneID 29369

Source Escherichia coli.

Molecular Weight 重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)Approximately 21.0 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

Quantity 5µg/25µg/1000µg

AA Sequence EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQTELQK PFKYTTVTKR SRRIRPTHPG

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.

Endotoxin Less than 1 EU/µg of rRtFGF-18 as determined by LAL method.

Reconstitution 重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)

SDS-PAGE

Reference 1. Itoh N, Ornitz DM. 2004. Trends Genet. 20:563-9.
2. Itoh N. 2007. Biol Pharm Bull. 30:1819-25.
3. Liu Z, Xu J, Colvin JS, et al. 2002. Genes Dev. 16:859-69.
4. Ohbayashi N, Shibayama M, Kurotaki Y, et al. 2002. Genes Dev. 16:870-9.
5. Usui H, Shibayama M, Ohbayashi N, et al. 2004. Biochem Biophys Res Commun. 322:887-92.
6. Ellsworth JL, Berry J, Bukowski T, et al. 2002. Osteoarthritis Cartilage. 10:308-20.
7. Moore EE, Bendele AM, Thompson DL, et al. 2005. Osteoarthritis Cartilage. 13:623-31.

Background Rat FGF-18 is encoded by the FGF18 gene in rats. By phylogenetic analysis and gene location analysis, FGF-18 is divided into FGF-8 subfamily which has three members FGF-8, FGF-17 and FGF-18. Using FGF knockout mice model, the numbers of this subfamily were testified that have crucial roles of in embryo development. FGF-18–/– mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF-18 also has significantroles in lung development and has an anabolic effect on cartilage formation. Additionally, it has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Rat FGF-18 shares 98 % amino acidsequence identity with human.

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Species	Rat
Accession	O88182
GeneID	29369
Source	Escherichia coli.
Molecular Weight	重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)Approximately 21.0 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
Quantity	5µg/25µg/1000µg
AA Sequence	EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQTELQK PFKYTTVTKR SRRIRPTHPG
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.
Endotoxin	Less than 1 EU/µg of rRtFGF-18 as determined by LAL method.
Reconstitution	重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)
SDS-PAGE
Reference	1. Itoh N, Ornitz DM. 2004. Trends Genet. 20:563-9. 2. Itoh N. 2007. Biol Pharm Bull. 30:1819-25. 3. Liu Z, Xu J, Colvin JS, et al. 2002. Genes Dev. 16:859-69. 4. Ohbayashi N, Shibayama M, Kurotaki Y, et al. 2002. Genes Dev. 16:870-9. 5. Usui H, Shibayama M, Ohbayashi N, et al. 2004. Biochem Biophys Res Commun. 322:887-92. 6. Ellsworth JL, Berry J, Bukowski T, et al. 2002. Osteoarthritis Cartilage. 10:308-20. 7. Moore EE, Bendele AM, Thompson DL, et al. 2005. Osteoarthritis Cartilage. 13:623-31.
Background	Rat FGF-18 is encoded by the FGF18 gene in rats. By phylogenetic analysis and gene location analysis, FGF-18 is divided into FGF-8 subfamily which has three members FGF-8, FGF-17 and FGF-18. Using FGF knockout mice model, the numbers of this subfamily were testified that have crucial roles of in embryo development. FGF-18–/– mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF-18 also has significantroles in lung development and has an anabolic effect on cartilage formation. Additionally, it has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Rat FGF-18 shares 98 % amino acidsequence identity with human.

產(chan)品名(ming)稱：重(zhong)組(zu)大(da)鼠成纖維(wei)細胞(bao)生(sheng)長因子(zi)18蛋(dan)白(bai)