重組大鼠(shu)角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai) - 上海康(kang)朗生(sheng)物科技有(you)限(xian)公(gong)司(si)

產(chan)品(pin)展示(shi)

產(chan)品(pin)詳情(qing)

產(chan)品(pin)名(ming)稱(cheng)：重(zhong)組大鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)
產(chan)品(pin)型號：rRtKGF-2/FGF-10
產(chan)品(pin)廠(chang)商(shang)：KALANG
產(chan)品(pin)文(wen)檔(dang)：

妳添加了1件(jian)商(shang)品(pin) 查看(kan)購(gou)物(wu)車(che)

簡單介(jie)紹：

重組(zu)大鼠(shu)角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)與(yu)其(qi)它公司(si)提供(gong)的重組(zu)蛋(dan)白(bai)不同，rRtKGF-2/FGF-10蛋(dan)白(bai)產(chan)品(pin)為(wei)采用CFS的(de)無細(xi)胞(bao)麥(mai)胚(pei)蛋(dan)白(bai)合(he)成系統(tong)表達出(chu)來的重(zhong)組蛋(dan)白(bai)，可表達出(chu)對細(xi)胞(bao)有(you)毒性(xing)、易被(bei)蛋(dan)白(bai)酶(mei)降(jiang)解的蛋(dan)白(bai)；並(bing)獲得具(ju)有(you)良好(hao)的可溶(rong)性，並(bing)有(you)翻(fan)譯(yi)後修飾、從而(er)部(bu)分(fen)具(ju)有(you)功能(neng)的蛋(dan)白(bai).同(tong)時獨有(you)的全(quan)自(zi)動蛋(dan)白(bai)純化(hua)技術則簡便(bian)高效(xiao)，將蛋(dan)白(bai)純化(hua)過(guo)程(cheng)中對(dui)蛋(dan)白(bai)的(de)損傷降低到(dao)*小程(cheng)度(du).重(zhong)組大鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)（全(quan)長序(xu)列）產(chan)品(pin)可用於(yu)Western Blot驗(yan)證(zheng)、抗體制(zhi)備(bei)、蛋(dan)白(bai)檢(jian)測、ELISA等(deng)試(shi)驗(yan)中.

詳情(qing)介紹：

重組(zu)大鼠(shu)角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)

Species Rat

Accession P70492

GeneID 25443

Source Escherichia coli.

Molecular Weight 重組(zu)大(da)鼠(shu)角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.

Quantity 5µg/25µg/1000µg

AA Sequence QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

Purity 重(zhong)組(zu)大(da)鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 × 10³ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 重(zhong)組(zu)大(da)鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.

Endotoxin Less than 1 EU/µg of rRtKGF-2/FGF-10 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組(zu)大(da)鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)

SDS-PAGE

Reference 1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.
2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.
3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.
4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.

Background Fibroblast growth factor 10 is a protein which belongs to the fibroblast growth factor (FGF) family and FGF family members are involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant rat FGF-10 contains a 179 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and murine FGF-10.

標(biao)題：

內(nei)容：

聯系人：

聯系電話(hua)：

Email：

公司(si)名(ming)稱(cheng)：

聯系地(di)址：

註(zhu)：1.可以使用快(kuai)捷鍵(jian)Alt+S或(huo)Ctrl+Enter發(fa)送(song)信息!

2.如有(you)必要(yao),請(qing)您(nin)留(liu)下您的(de)詳細(xi)聯系方式!

滬公網(wang)安(an)備(bei) 31011702004399號

Species	Rat
Accession	P70492
GeneID	25443
Source	Escherichia coli.
Molecular Weight	重組(zu)大(da)鼠(shu)角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.
Quantity	5µg/25µg/1000µg
AA Sequence	QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
Purity	重(zhong)組(zu)大(da)鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 × 10³ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重(zhong)組(zu)大(da)鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.
Endotoxin	Less than 1 EU/µg of rRtKGF-2/FGF-10 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組(zu)大(da)鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)
SDS-PAGE
Reference	1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4. 2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404. 3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63. 4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.
Background	Fibroblast growth factor 10 is a protein which belongs to the fibroblast growth factor (FGF) family and FGF family members are involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant rat FGF-10 contains a 179 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and murine FGF-10.

產(chan)品(pin)名(ming)稱(cheng)：重(zhong)組大鼠角(jiao)質(zhi)形成細(xi)胞(bao)生(sheng)長因子(zi)-2 / FGF-10蛋(dan)白(bai)