重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai) - 上(shang)海(hai)康(kang)朗(lang)生(sheng)物(wu)科技有限(xian)公(gong)司(si)

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產品(pin)名(ming)稱(cheng)：重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)
產(chan)品(pin)型(xing)號(hao)：rRtFGF-9
產品(pin)廠商(shang)：KALANG
產(chan)品文(wen)檔(dang)：

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重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)與(yu)其(qi)它(ta)公(gong)司(si)提(ti)供的重(zhong)組蛋(dan)白(bai)不(bu)同(tong)，rRtFGF-9蛋(dan)白(bai)產(chan)品(pin)為(wei)采用(yong)CFS的無(wu)細(xi)胞(bao)麥胚(pei)蛋(dan)白(bai)合成(cheng)系(xi)統表(biao)達出來的重(zhong)組蛋(dan)白(bai)，可(ke)表(biao)達出對(dui)細(xi)胞(bao)有毒(du)性、易(yi)被(bei)蛋(dan)白(bai)酶(mei)降(jiang)解的(de)蛋(dan)白(bai)；並獲得(de)具有良好(hao)的(de)可溶(rong)性，並有翻(fan)譯後修(xiu)飾(shi)、從而(er)部分(fen)具有功(gong)能(neng)的(de)蛋(dan)白(bai).同(tong)時(shi)獨有的(de)全(quan)自(zi)動(dong)蛋(dan)白(bai)純(chun)化技術則簡(jian)便(bian)高(gao)效(xiao)，將蛋(dan)白(bai)純(chun)化過(guo)程中(zhong)對(dui)蛋(dan)白(bai)的(de)損傷降(jiang)低(di)到*小(xiao)程度(du).重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)（全(quan)長序列）產(chan)品可(ke)用(yong)於Western Blot驗證、抗體(ti)制備、蛋(dan)白(bai)檢(jian)測(ce)、ELISA等(deng)試(shi)驗中(zhong).

詳(xiang)情(qing)介(jie)紹：

重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)

Synonyms GAF, Glia-activating factor, HBGF-9

Species Rat

Accession P36364

GeneID 25444

Source Escherichia coli.

Molecular Weight 重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)Approximately 23.1 kDa, a single non-glycosylated polypeptide chain containing 205 amino acids.

Quantity 5µg/20µg/1000µg

AA Sequence LGEVGSYFGV QDAVPFGNVP VLPVDSPVLL SDHLGQSEAG GLPRGPAVTD LDHLKGILRR RQLYCRTGFH LEIFPNGTIQ GTRKDHSRFG ILEFISIAVG LVSIRGVDSG LYLGMNEKGE LYGSEKLTQE CVFREQFEEN WYNTYSSNLY KHVDTGRRYY VALNKDGTPR EGTRTKRHQK FTHFLPRPVD PDKVPELYKD ILSQS

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH 8.0.

Endotoxin Less than 1 EU/µg of rRtFGF-9-1 as determined by LAL method.

Reconstitution 重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)

SDS-PAGE

Reference 1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.
2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.
3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.

Background Fibroblast growth factor-9 (FGF-9) is a member of the fibroblast growth factor (FGF) family. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. The rat FGF-9 shares 98 % a.a. sequence identity with human FGF-9.

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Synonyms	GAF, Glia-activating factor, HBGF-9
Species	Rat
Accession	P36364
GeneID	25444
Source	Escherichia coli.
Molecular Weight	重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)Approximately 23.1 kDa, a single non-glycosylated polypeptide chain containing 205 amino acids.
Quantity	5µg/20µg/1000µg
AA Sequence	LGEVGSYFGV QDAVPFGNVP VLPVDSPVLL SDHLGQSEAG GLPRGPAVTD LDHLKGILRR RQLYCRTGFH LEIFPNGTIQ GTRKDHSRFG ILEFISIAVG LVSIRGVDSG LYLGMNEKGE LYGSEKLTQE CVFREQFEEN WYNTYSSNLY KHVDTGRRYY VALNKDGTPR EGTRTKRHQK FTHFLPRPVD PDKVPELYKD ILSQS
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH 8.0.
Endotoxin	Less than 1 EU/µg of rRtFGF-9-1 as determined by LAL method.
Reconstitution	重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)
SDS-PAGE
Reference	1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9. 2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31. 3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.
Background	Fibroblast growth factor-9 (FGF-9) is a member of the fibroblast growth factor (FGF) family. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. The rat FGF-9 shares 98 % a.a. sequence identity with human FGF-9.

產品(pin)名(ming)稱(cheng)：重(zhong)組大(da)鼠(shu)成(cheng)纖(xian)維細(xi)胞(bao)生(sheng)長因子9蛋(dan)白(bai)