重(zhong)組大鼠(shu)midkine蛋白(bai) - 上海(hai)康朗(lang)生(sheng)物科技(ji)有(you)限公(gong)司

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產品(pin)名(ming)稱(cheng)：重(zhong)組大鼠(shu)midkine蛋白(bai)
產品(pin)型號：rRtMidkine
產品(pin)廠商：KALANG
產品(pin)文檔(dang)：

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簡單(dan)介(jie)紹：

重(zhong)組大鼠(shu)midkine蛋白(bai)與其(qi)它公(gong)司提供的重(zhong)組蛋白(bai)不同，rRtMidkine蛋白(bai)產品(pin)為(wei)采用(yong)CFS的無(wu)細(xi)胞麥(mai)胚蛋白(bai)合(he)成系(xi)統表達出(chu)來的重(zhong)組蛋白(bai)，可(ke)表達出(chu)對(dui)細(xi)胞有(you)毒性、易(yi)被(bei)蛋白(bai)酶(mei)降(jiang)解(jie)的蛋白(bai)；並(bing)獲得具有(you)良好的可(ke)溶(rong)性，並(bing)有(you)翻譯(yi)後(hou)修(xiu)飾(shi)、從而部分具(ju)有(you)功(gong)能(neng)的蛋白(bai).同時(shi)獨(du)有(you)的全自(zi)動蛋白(bai)純(chun)化(hua)技(ji)術則(ze)簡便高(gao)效，將(jiang)蛋白(bai)純(chun)化(hua)過(guo)程中(zhong)對(dui)蛋白(bai)的損傷(shang)降(jiang)低到*小(xiao)程度(du).重(zhong)組大鼠(shu)midkine蛋白(bai)（全長(chang)序列）產品(pin)可(ke)用(yong)於Western Blot驗證、抗(kang)體制(zhi)備、蛋白(bai)檢(jian)測(ce)、ELISA等試(shi)驗中(zhong).

詳(xiang)情(qing)介(jie)紹：

重(zhong)組大鼠(shu)midkine蛋白(bai)

Species Rat

Accession Q9R1S9

GeneID 81517

Source Escherichia coli.

Molecular Weight 重(zhong)組大鼠(shu)midkine蛋白(bai)Approximately 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.

Quantity 5µg/20µg/1000µg

AA Sequence VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD

Purity 重(zhong)組大鼠(shu)midkine蛋白(bai)> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.

Endotoxin 重(zhong)組大鼠(shu)midkine蛋白(bai)Less than 0.1 EU/μg of rRtMidkine as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重(zhong)組大鼠(shu)midkine蛋白(bai)

SDS-PAGE

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Species	Rat
Accession	Q9R1S9
GeneID	81517
Source	Escherichia coli.
Molecular Weight	重(zhong)組大鼠(shu)midkine蛋白(bai)Approximately 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.
Quantity	5µg/20µg/1000µg
AA Sequence	VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD
Purity	重(zhong)組大鼠(shu)midkine蛋白(bai)> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
Endotoxin	重(zhong)組大鼠(shu)midkine蛋白(bai)Less than 0.1 EU/μg of rRtMidkine as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重(zhong)組大鼠(shu)midkine蛋白(bai)
SDS-PAGE

產品(pin)名(ming)稱(cheng)：重(zhong)組大鼠(shu)midkine蛋白(bai)