產(chan)品名稱(cheng)：人8-異(yi)構(gou)前(qian)列腺(xian)素(su)F2α(8-epi-PGF2α)化學(xue)發光(guang)**分析(xi)試(shi)劑(ji)盒

人8-異(yi)構(gou)前(qian)列腺(xian)素(su)F2α(8-epi-PGF2α)化學(xue)發光(guang)**分析(xi)試(shi)劑(ji)盒

基本原理：

本(ben)試(shi)劑(ji)盒采(cai)用競爭CLIA法(fa)。用(yong)人8-epi-PGF2α抗原包(bao)被於(yu)酶標板上，實驗時樣品或標(biao)準(zhun)品中(zhong)的(de)8-epi-PGF2α與(yu)包(bao)被的(de)8-epi-PGF2α競爭生物素(su)標記(ji)的(de)抗人8-epi-PGF2α單抗上的(de)結(jie)合(he)位(wei)點(dian)，遊離(li)的(de)成分(fen)被洗(xi)去。加(jia)入(ru)辣根過(guo)氧(yang)化(hua)物酶(mei)標(biao)記(ji)的(de)親和素(su)，生物素(su)與親和素(su)特異(yi)性(xing)結(jie)合(he)而(er)形(xing)成**復(fu)合(he)物，遊離(li)的(de)成分(fen)被洗(xi)去。加(jia)入(ru)發(fa)光(guang)底(di)物混合液，發光(guang)底(di)物在(zai)辣根過(guo)氧(yang)化(hua)物酶(mei)的(de)催(cui)化下(xia)發(fa)出(chu)熒光(guang)，用化(hua)學(xue)發光(guang)**分析(xi)儀測(ce)定(ding)化(hua)學(xue)發光(guang)值（RLU），8-epi-PGF2α濃(nong)度(du)與化學(xue)發光(guang)值之(zhi)間呈負(fu)相(xiang)關，通過(guo)繪(hui)制標準(zhun)曲(qu)線計算(suan)出樣(yang)品中(zhong)8-epi-PGF2α的(de)濃(nong)度。

人8-異(yi)構(gou)前(qian)列腺(xian)素(su)F2α(8-epi-PGF2α)化學(xue)發光(guang)**分析(xi)試(shi)劑(ji)盒描(miao)述(shu)：

英文名稱(cheng)	Human 8-epi-PGF2α (8-Epi-Prostaglandin F2 Alpha) CLIA Kit
中(zhong)文名稱(cheng)	人8-異(yi)構(gou)前(qian)列腺(xian)素(su)F2α(8-epi-PGF2α)化學(xue)發光(guang)**分析(xi)試(shi)劑(ji)盒
貨(huo)號	KL-H0199c	種屬(shu)	Human/人
規(gui)格(ge)	96T/Kit (8*12 wells)
檢測(ce)方法(fa)	競爭法(fa)
檢(jian)測(ce)範(fan)圍(wei)	7.813~500pg/mL	靈(ling)敏(min)度	4.688pg/mL

人8-異(yi)構(gou)前(qian)列腺(xian)素(su)F2α(8-epi-PGF2α)化學(xue)發光(guang)**分析(xi)試(shi)劑(ji)盒試(shi)劑(ji)盒組(zu)成：

中(zhong)文名稱(cheng)	英文名稱(cheng)	規(gui)格(ge)	保(bao)存
CLIA酶標板	Micro CLIA Plate	8×12 wells	4℃/-20℃ #
凍(dong)幹(gan)標準(zhun)品	Reference Standard	2 支	4℃/-20℃ #
標準(zhun)品&樣(yang)品稀(xi)釋液	Reference Standard & Sample Diluent	1瓶 20mL	4℃
濃(nong)縮(suo)生物素(su)化抗體	Concentrated Biotinylated Detection Ab	1支 120μL	4℃/-20℃ #
生物素(su)化抗體稀(xi)釋液	Biotinytated Detection Ab Diluent	1瓶10mL	4℃
濃(nong)縮(suo)HRP酶結(jie)合(he)物	Concentrated HRP Conjugate	1支 120μL	4℃(避光)
酶結(jie)合(he)物稀(xi)釋液	HRP Conjugate Diluent	1瓶 10mL	4℃
濃(nong)縮(suo)洗滌液（25×）	Concentrated Wash Buffer (25×)	1瓶 30mL	4℃
發(fa)光(guang)底物A液	Substrate Reagent A	1瓶 5mL	4℃(避(bi)光(guang))
發光底(di)物B液	Substrate Reagent B	1瓶 5mL	4℃(避(bi)光(guang))
封板覆(fu)膜	Plate Sealer	5 張(zhang)
產(chan)品說(shuo)明(ming)書(shu)	Manual	1 份(fen)
質檢報告	Certificate of Analysis	1 份(fen)
特別(bie)說明(ming)： #: 壹(yi)周(zhou)內(nei)使用(yong)可(ke)存於4℃，需(xu)長(chang)時間存放(fang)或多次(ci)使用(yong)建(jian)議(yi)存於-20℃.

人8-異(yi)構(gou)前(qian)列腺(xian)素(su)F2α(8-epi-PGF2α)化學(xue)發光(guang)**分析(xi)試(shi)劑(ji)盒操作(zuo)步驟(zhou)：

1.在各(ge)孔(kong)中(zhong)加(jia)入(ru)標(biao)準(zhun)品或樣(yang)品各(ge) 100μL，37℃孵育(yu)90分鐘(zhong)
2. 倒去(qu)孔(kong)內液體，拍(pai)幹(gan)，加(jia)入(ru) 100μL  生物素(su)化抗體工(gong)作(zuo)液，37℃孵育(yu) 60 分鐘(zhong)
3. 洗(xi)滌(di) 3 次(ci)
4. 加(jia)入(ru) 100μL 酶(mei)結(jie)合(he)物工(gong)作(zuo)液， 37℃孵育(yu) 30 分鐘(zhong)
5. 洗(xi)滌(di) 5 次(ci)
6. 加(jia)入(ru) 100μL 發(fa)光(guang)底(di)物混合液， 37℃孵育(yu) 5 分鐘(zhong)左(zuo)右(you)
7. 立(li)即測(ce)定(ding)各(ge)孔(kong)的(de)化(hua)學(xue)發光(guang)值
8. 結(jie)果(guo)計(ji)算(suan)

KL-IP10-b 牛(niu)10kDa幹(gan)擾素(su)γ誘導蛋(dan)白(IP10) 檢測(ce)試(shi)劑(ji)盒 Bovine Interferon Gamma Induced Protein 10kDa (IP10) ELISA Kit Bos taurus; Bovine  Cattle 0.156-10ng/m 0.058ng/m 96T 12 months
KL-PIGR-b 牛(niu)多聚(ju)**球(qiu)蛋(dan)白受體(ti)(PIGR) 檢(jian)測(ce)試(shi)劑(ji)盒 Bovine Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit Bos taurus; Bovine  Cattle 0.625-40ng/mL 0.238ng/mL 96T 12 months
KL-S100A8-b 牛(niu)S100鈣(gai)結(jie)合(he)蛋(dan)白A8(S100A8) 檢測(ce)試(shi)劑(ji)盒 Bovine S100 Calcium Binding Protein A8 (S100A8) ELISA Kit Bos taurus; Bovine  Cattle 78.125-5000pg/mL 32pg/mL 96T 12 months
KL-S100A9-b 牛(niu)S100鈣(gai)結(jie)合(he)蛋(dan)白A9(S100A9) 檢測(ce)試(shi)劑(ji)盒 Bovine S100 Calcium Binding Protein A9 (S100A9) ELISA Kit Bos taurus; Bovine  Cattle 0.313-20ng/mL 0.102ng/mL 96T 12 months
KL-ITaC-b 牛(niu)幹(gan)擾素(su)誘導T-細(xi)胞(bao)α亞族趨化劑(ji)(ITαC) 檢(jian)測(ce)試(shi)劑(ji)盒 Bovine Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit Bos taurus; Bovine  Cattle 46.875-3000pg/mL 15.46pg/mL 96T 12 months
KL-SATB1-Hu 人SATB同源(yuan)框蛋(dan)白1(SATB1) 檢測(ce)試(shi)劑(ji)盒 human SATB Homeobox Protein 1 (SATB1) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.102ng/mL 96T 12 months
KL-LDHC-Hu 人乳(ru)酸(suan)脫氫酶(mei)C(LDHC) 檢(jian)測(ce)試(shi)劑(ji)盒 human Lactate Dehydrogenase C (LDHC) ELISA Kit Homo sapiens human 1.563-100ng/mL 0.54ng/mL 96T 12 months
KL-CUZD1-Hu 人CUB帶(dai)狀瘡(chuang)疹(zhen)透(tou)明(ming)區樣(yang)域蛋(dan)白1(CUZD1) 檢測(ce)試(shi)劑(ji)盒 human CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.114ng/mL 96T 12 months
KL-CECR1-Hu 人貓(mao)眼(yan)綜合(he)征染(ran)色(se)體區候(hou)選(xuan)基因1(CECR1) 檢測(ce)試(shi)劑(ji)盒 human Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.115ng/mL 96T 12 months
KL-GAS6-Hu 人生長(chang)停(ting)滯(zhi)特異(yi)性(xing)蛋(dan)白6(GAS6) 檢測(ce)試(shi)劑(ji)盒 human Growth Arrest Specific Protein 6 (GAS6) ELISA Kit Homo sapiens human 6.25-400pg/mL 2.45pg/mL 96T 12 months
KL-SEMA3E-Hu 人信號素(su)3E(SEMA3E) 檢測(ce)試(shi)劑(ji)盒 human Semaphorin 3E (SEMA3E) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.116ng/mL 96T 12 months
KL-AT-Hu 人抗凝血酶(AT) 檢(jian)測(ce)試(shi)劑(ji)盒 human Antithrombin (AT) ELISA Kit Homo sapiens human 31.25-2000ng/mL 12.6ng/mL 96T 12 months
KL-VIL1-Hu 人絨(rong)毛蛋(dan)白1(VIL1) 檢測(ce)試(shi)劑(ji)盒 human Villin 1 (VIL1) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.054ng/mL 96T 12 months
KL-PSCA-Hu 人前(qian)列腺(xian)幹(gan)細胞抗原(PSCA) 檢(jian)測(ce)試(shi)劑(ji)盒 human Prostate Stem Cell Antigen (PSCA) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.055ng/mL 96T 12 months