產(chan)品(pin)名(ming)稱：人11去氫血(xue)栓烷B2(11-DH-TXB2)化(hua)學(xue)發(fa)光**分析(xi)試(shi)劑(ji)盒

人(ren)11去氫血(xue)栓烷B2(11-DH-TXB2)化(hua)學(xue)發(fa)光**分析(xi)試(shi)劑(ji)盒

基(ji)本(ben)原(yuan)理：

本試(shi)劑(ji)盒采(cai)用(yong)競(jing)爭(zheng)CLIA法。用(yong)人(ren)11-DH-TXB2抗原(yuan)包被於(yu)酶標(biao)板上(shang)，實驗時樣(yang)品(pin)或標(biao)準(zhun)品(pin)中的11-DH-TXB2與包被的(de)11-DH-TXB2競爭(zheng)生物素(su)標(biao)記的(de)抗人(ren)11-DH-TXB2單抗上的(de)結(jie)合位(wei)點，遊離的(de)成分被洗(xi)去。加入(ru)辣(la)根過(guo)氧(yang)化(hua)物(wu)酶標(biao)記的(de)親和素(su)，生(sheng)物(wu)素(su)與親和素(su)特異(yi)性(xing)結(jie)合而(er)形成(cheng)**復(fu)合物(wu)，遊離的(de)成分被洗(xi)去。加入(ru)發(fa)光底物(wu)混合液(ye)，發(fa)光底物(wu)在(zai)辣(la)根過(guo)氧(yang)化(hua)物(wu)酶的催(cui)化下發(fa)出(chu)熒(ying)光，用(yong)化(hua)學(xue)發(fa)光**分析(xi)儀(yi)測定(ding)化(hua)學(xue)發(fa)光值(zhi)（RLU），11-DH-TXB2濃(nong)度(du)與化學(xue)發(fa)光值(zhi)之(zhi)間(jian)呈(cheng)負相關，通(tong)過(guo)繪(hui)制標(biao)準(zhun)曲線(xian)計(ji)算(suan)出(chu)樣(yang)品(pin)中11-DH-TXB2的濃(nong)度(du)。

人(ren)11去氫血(xue)栓烷B2(11-DH-TXB2)化(hua)學(xue)發(fa)光**分析(xi)試(shi)劑(ji)盒描(miao)述(shu)：

英(ying)文名(ming)稱	Human 11-DH-TXB2 (11-Dehydro-thromboxane B2) CLIA Kit
中文名(ming)稱	人11去氫血(xue)栓烷B2(11-DH-TXB2)化(hua)學(xue)發(fa)光**分析(xi)試(shi)劑(ji)盒
貨(huo)號(hao)	KL-H0187c	種(zhong)屬(shu)	Human/人(ren)
規(gui)格(ge)	96T/Kit (8*12 wells)
檢(jian)測方(fang)法	競(jing)爭(zheng)法
檢(jian)測範(fan)圍	7.813~500pg/mL	靈(ling)敏(min)度	4.688pg/mL

人(ren)11去氫血(xue)栓烷B2(11-DH-TXB2)化(hua)學(xue)發(fa)光**分析(xi)試(shi)劑(ji)盒試(shi)劑(ji)盒組(zu)成(cheng)：

中文名(ming)稱	英文名(ming)稱	規格(ge)	保存(cun)
CLIA酶標(biao)板	Micro CLIA Plate	8×12 wells	4℃/-20℃ #
凍(dong)幹標(biao)準(zhun)品(pin)	Reference Standard	2 支(zhi)	4℃/-20℃ #
標(biao)準(zhun)品(pin)&樣品(pin)稀釋(shi)液(ye)	Reference Standard & Sample Diluent	1瓶 20mL	4℃
濃(nong)縮(suo)生(sheng)物素(su)化(hua)抗(kang)體(ti)	Concentrated Biotinylated Detection Ab	1支(zhi) 120μL	4℃/-20℃ #
生物素(su)化(hua)抗(kang)體(ti)稀釋(shi)液(ye)	Biotinytated Detection Ab Diluent	1瓶10mL	4℃
濃(nong)縮(suo)HRP酶結合物(wu)	Concentrated HRP Conjugate	1支(zhi) 120μL	4℃(避光)
酶結合物(wu)稀釋(shi)液(ye)	HRP Conjugate Diluent	1瓶 10mL	4℃
濃(nong)縮(suo)洗(xi)滌(di)液(ye)（25×）	Concentrated Wash Buffer (25×)	1瓶 30mL	4℃
發(fa)光底物(wu)A液(ye)	Substrate Reagent A	1瓶 5mL	4℃(避光)
發(fa)光底物(wu)B液(ye)	Substrate Reagent B	1瓶 5mL	4℃(避光)
封(feng)板(ban)覆(fu)膜	Plate Sealer	5 張(zhang)
產(chan)品(pin)說明(ming)書(shu)	Manual	1 份
質(zhi)檢(jian)報(bao)告(gao)	Certificate of Analysis	1 份
特別(bie)說明(ming)： #: 壹(yi)周內(nei)使(shi)用(yong)可存(cun)於(yu)4℃，需長時間(jian)存(cun)放或多次(ci)使(shi)用(yong)建(jian)議(yi)存(cun)於(yu)-20℃.

人(ren)11去氫血(xue)栓烷B2(11-DH-TXB2)化(hua)學(xue)發(fa)光**分析(xi)試(shi)劑(ji)盒操(cao)作(zuo)步(bu)驟(zhou)：

1.在(zai)各(ge)孔中加入(ru)標(biao)準(zhun)品(pin)或樣(yang)品(pin)各(ge) 100μL，37℃孵育(yu)90分鐘
2. 倒去孔內(nei)液(ye)體，拍幹，加入(ru) 100μL  生物(wu)素(su)化(hua)抗(kang)體(ti)工(gong)作液(ye)，37℃孵育(yu) 60 分鐘
3. 洗(xi)滌(di) 3 次(ci)
4. 加入(ru) 100μL 酶結合物(wu)工(gong)作液(ye)， 37℃孵育(yu) 30 分鐘
5. 洗(xi)滌(di) 5 次(ci)
6. 加入(ru) 100μL 發(fa)光底物(wu)混合液(ye)， 37℃孵育(yu) 5 分鐘左右(you)
7. 立(li)即測定(ding)各(ge)孔的化(hua)學(xue)發(fa)光值(zhi)
8. 結(jie)果(guo)計(ji)算(suan)

KL-FTL-Ra 大鼠(shu)輕肽(tai)鐵(tie)蛋(dan)白(bai)(FTL) 檢(jian)測試(shi)劑(ji)盒 Rat Ferritin, Light Polypeptide (FTL) ELISA Kit Rattus norvegicus  Rat 0.156-10ng/mL 0.051ng/mL 96T 12 months
KL-SIRT6-Hu 人(ren)沈默調節(jie)蛋(dan)白(bai)6(SIRT6) 檢(jian)測試(shi)劑(ji)盒 human Sirtuin 6 (SIRT6) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.124ng/mL 96T 12 months
KL-CTNNb1-Ra 大鼠(shu)連(lian)環蛋(dan)白(bai)β1(CTNNβ1) 檢(jian)測試(shi)劑(ji)盒 Rat Catenin Beta 1 (CTNNb1) ELISA Kit Rattus norvegicus  Rat 0.156-10ng/mL 0.065ng/mL 96T 12 months
KL-CAPN1-Hu 人(ren)鈣(gai)蛋(dan)白(bai)酶1(CAPN1) 檢(jian)測試(shi)劑(ji)盒 human Calpain 1, Large Subunit (CAPN1) ELISA Kit Homo sapiens human 1.563-100ng/mL 0.58ng/mL 96T 12 months
KL-GSK3b-Ra 大鼠(shu)糖原(yuan)合酶激酶3β(GSK3β) 檢(jian)測試(shi)劑(ji)盒 Rat Glycogen Synthase Kinase 3 Beta (GSK3b) ELISA Kit Rattus norvegicus  Rat 0.781-50ng/mL 0.35ng/mL 96T 12 months
KL-TOMM70A-Hu 人(ren)線(xian)粒(li)體外膜轉(zhuan)位(wei)酶70A(TOMM70A) 檢(jian)測試(shi)劑(ji)盒 human Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.101ng/mL 96T 12 months
KL-TTR-p 豬(zhu)轉(zhuan)甲(jia)狀腺(xian)素(su)蛋(dan)白(bai)(TTR) 檢(jian)測試(shi)劑(ji)盒 porcine Transthyretin (TTR) ELISA Kit Sus scrofa; Porcine  Pig 1.563-100ng/mL 0.68ng/mL 96T 12 months
KL-SNX13-Hu 人(ren)分揀連(lian)接(jie)蛋(dan)白(bai)13(SNX13) 檢(jian)測試(shi)劑(ji)盒 human Sorting Nexin 13 (SNX13) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.128ng/mL 96T 12 months
KL-SNX17-Hu 人(ren)分揀連(lian)接(jie)蛋(dan)白(bai)17(SNX17) 檢(jian)測試(shi)劑(ji)盒 human Sorting Nexin 17 (SNX17) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.057ng/mL 96T 12 months
KL-MFAP4-Hu 人(ren)微(wei)纖(xian)絲關(guan)聯(lian)蛋(dan)白(bai)4(MFAP4) 檢(jian)測試(shi)劑(ji)盒 human Microfibrillar Associated Protein 4 (MFAP4) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.148ng/mL 96T 12 months
KL-CDNF-Ra 大鼠(shu)腦(nao)多巴(ba)胺神經(jing)營養因(yin)子(zi)(CDNF) 檢(jian)測試(shi)劑(ji)盒 Rat Cerebral Dopamine Neurotrophic Factor (CDNF) ELISA Kit Rattus norvegicus  Rat 0.156-10ng/mL 0.067ng/mL 96T 12 months
KL-TREM2-Mu 小(xiao)鼠(shu)髓(sui)細(xi)胞(bao)觸發(fa)受(shou)體2(TREM2)  檢(jian)測試(shi)劑(ji)盒 mouse Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit Mus musculus  mouse 78.125-5000pg/mL 23.8pg/mL 96T 12 months
KL-PDCD6-Hu 人(ren)細(xi)胞(bao)程序性(xing)死(si)亡(wang)蛋(dan)白(bai)6(PDCD6) 檢(jian)測試(shi)劑(ji)盒 human Programmed Cell Death Protein 6 (PDCD6) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.118ng/mL 96T 12 months
KL-PDCD6IP-Hu 人(ren)細(xi)胞(bao)程序性(xing)死(si)亡(wang)蛋(dan)白(bai)6相互作用(yong)蛋(dan)白(bai)(PDCD6IP) 檢(jian)測試(shi)劑(ji)盒 human Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.107ng/mL 96T 12 months